1.2.5 Anatomy, Physiology, and Health Education I Laboratory
Lab 4 Estimation of Haemoglobin with the help of Sahli's Haemoglobinometer (Cont'd...)
Hb count by Sahli's haemoglobinometer (acid haematin method):
Principle: Anticoagulated blood is added to the 0.1 N HCl and kept for 5-7 minutes to form acid haematin. The color of this acid haematin should be matched with the solution, present in the calibration tube. Distilled water is added to the acid haematin until the color matches and the final reading is directly noted from the graduation in the calibration tube. [Please note that 100 percent on the scale corresponds to 14.5gm % to 15gm %].
Requirements: Sahli's haemoglobinometer, Hydrochloric acid, distilled water.
Procedure: Place N/10 HCL in diluting tube up to the mark 20. Take blood in the haemoglobin pipette up to 20-cubic-mm-mark and blow it into diluting tube and rinse well. After 10 minutes add distilled water in drops and mix the tube until it has exactly the same color as the comparison standards. Note the reading, which indicates the
percentage of haemoglobin.
The Hb estimation of the given sample is ..... g/100 ml of blood/.....g/dl of blood/.....G%.
i. Pipetting of blood should be done cautiously
ii. Mix the blood properly with HCl by using stirrer
iii. Match the color cautiously.
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Estimation of Haemoglobin with Cyanmethemoglobin (Drabkin's) method