Electrophoresis Quotes (2 quotes)
Of the nucleosides from deoxyribonucleic acids, all that was known with any certainty [in the 1940s] was that they were 2-deoxy-D-ribosides of the bases adenine, guanine, thymine and cytosine and it was assumed that they were structurally analogous to the ribonucleosides. The chemistry of the nucleotides—the phosphates of the nucleosides—was in a correspondingly primitive state. It may well be asked why the chemistry of these groups of compounds was not further advanced, particularly since we recognize today that they occupy a central place in the history of the living cell. True, their full significance was for a long time unrecognized and emerged only slowly as biochemical research got into its stride but I think a more important reason is to be found in the physical properties of compounds of the nucleotide group. As water-soluble polar compounds with no proper melting points they were extremely difficult to handle by the classic techniques of organic chemistry, and were accordingly very discouraging substances to early workers. It is surely no accident that the major advances in the field have coincided with the appearance of new experimental techniques such as paper and ion-exchange chromatography, paper electrophoresis, and countercurrent distribution, peculiarly appropriate to the compounds of this group.
The century of biology upon which we are now well embarked is no matter of trivialities. It is a movement of really heroic dimensions, one of the great episodes in man’s intellectual history. The scientists who are carrying the movement forward talk in terms of nucleo-proteins, of ultracentrifuges, of biochemical genetics, of electrophoresis, of the electron microscope, of molecular morphology, of radioactive isotopes. But do not be misled by these horrendous terms, and above all do not be fooled into thinking this is mere gadgetry. This is the dependable way to seek a solution of the cancer and polio problems, the problems of rheumatism and of the heart. This is the knowledge on which we must base our solution of the population and food problems. This is the understanding of life.