Figure 7: Monte-Carlo simulation of HMM-propelled actin filament sliding ( μm) without guiding but with the same initial position and sliding direction for all filaments. (a) Distribution of sliding path lengths from onset of motility until the filament, for the first time, moves below red circle arc in inset. Inset: all 200 simulated filament paths starting on the red circular border at the starting point of the arrow. The direction of the latter represents the initial sliding direction. (b) Cumulative frequency distribution corresponding to frequency distribution in (a). Inset: a limited ensemble of 10 simulated filament paths clearly illustrating that also filaments that have moved toward the circle centre tend to rapidly reach and cross the circular border.