Washington: Scientists have genetically engineered a clone of the Zika virus strain for the first time, an advance that may aid the development of vaccine and therapies against the infection, linked to birth defects, such as microcephaly and Guillain-Barre syndrome
Cloning the virus unlocks scientists' ability to more quickly develop countermeasures and explore the evolution of the virus.
The researchers from the University of Texas Medical Branch (UTMB) constructed the Zika virus clone. Five fragments spanning the complete viral genome were cloned and assembled into the full-length clone of the Zika virus.
The infectious complementary DNA (cDNA) clone allows researchers to make Zika virus from test tube and cells on petri dishes. The researchers then used the UTMB-developed Zika mouse model to demonstrate that the cloned virus infected the mice and gave them neurological disease.
The team fed Aedes aegypti mosquitoes, one of the types known to transmit Zika, with human blood infected with either the parental Zika virus or the "human-made" virus and found the number of infected mosquitoes was similar.
These findings confirm that the cloned virus is highly infectious for Aedes aegypti mosquitoes. In addition, the results demonstrated that Aedes aegypti might be a good mosquito vector for Zika virus transmission. Furthermore, the team engineered a luciferase reporter Zika virus. Luciferase is the chemical in fireflies that gives them their signature glow.