Study of local anaesthetics by different methods
To study the local anaesthetic property of procaine HCL using nerve block anaesthesia by frog foot withdrawal method.
Local anaesthetics reversibly block impulse conduction along nerve axons and excitable membranes. Their action is used to produce local or regional anaesthesia and for blocking pain sensation. the local anaesthetic property can be easily studied by using
- Nerve block anaesthesia
- Surface anaesthesia
- Infiltration anaesthesia
Drugs: Procaine HCL (1%w/v)
Equipment: Frog board surgical instruments
- Decereb the frog and Place the frog (100-150 gm) and destroy the upper part of the spinal cord with the help of a pithing needle.
- Cut open the abdomen and remove all the abdominal organs, so that a pouch is made of abdominal walls.
- Expose the spinal nerves in the cavity
- Put the frog on the frog board with two of its hind legs hanging free from the board. Alternatively, the frog may be pinned to the board placed vertically so that its hind legs hang free
- Immense the right hind leg in the beaker containing 0.1N HCL.
- Note the brisk reflex withdrawal of the leg
- Wash the immerse leg with flowing water or normal saline.
- Repeat the same with the left hind leg.
- Wash the immersed left.
- Place about 10 ml of 1% w/v procaine Hcl in the abdominal pouch. The sciatic nerve flex is exposed to the local anaesthetic actions of the drugs. allow the drug to act for 5 min.
- Introduction to experimental pharmacology.
- Commonly used instruments in experimental pharmacology.
- Study of common laboratory animals.
- Maintenance of laboratory animals as per CPCSEA guidelines.
- Common laboratory techniques.
- Study of different routes of drugs administration in mice/rats.
- Study effect of hepatic microsomal enzyme inducers on phenobarbitone sleeping time in mice.
- Effect of drugs on ciliary motility of frog oesophagus
- Effect of drugs on rabbit eye.
- Effects of skeletal muscle relaxants using rota-rod apparatus.
- Effect of drugs on locomotor activity using actophotometer.
- Anticonvulsant effect of drugs by MES and PTZ method.
- Study of stereotype and anti-catatonic activity of drugs on rats/mice.
- Study of anxiolytic activity of drugs using rats/mice.
- Study of local anesthetics by different methods
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