Monoclonal antibodies represent identical immunoglobulins that are generated from a single parent immune B cell. Rabbit monoclonal antibodies refer to antibodies developed in rabbits, not mice, which are most often used to enhance antibodies.
Why do you need rabbit monoclonal antibodies?
Rabbits offer a unique advantage over mice in monoclonal antibody production, as they can produce large numbers of B cells and thus antibody structure. In addition, the rabbit immune system can produce antibodies with a 10 to 100-fold higher affinity for the target antigen than mouse monoclonal antibodies.
You can also check out the Boster Bio featured products to get the best rabbit monoclonal antibody products.
Image Source: Google
Reduce background noise
Rabbit antibodies can only detect one epitope, so the possibility of cross-reactivity with other proteins is low. It has also been observed that rabbit antibodies can bind to target antigens with greater affinity, resulting in a higher signal-to-noise ratio for rabbit antibodies compared to mouse antibodies. In this way, Rabbit mAabs creates a more specific and sensitive image – especially when there is a lot of background noise.
Increased specificity and high affinity
Mouse antibodies are highly specific; however, they could not detect small changes in the epitope or different versions of the protein. However, rabbit antibodies are highly specific and even recognize the cleaved version of the target protein.
The presence of high affinity antibodies can ensure that high signal strength can be achieved even under tight conditions. It produces clear images with high specificity and low background effects.
Suitable for immunohistochemistry
As rabbit monoclonal antibodies offer high sensitivity with little loss of specificity, they are an ideal choice for applications such as immunohistochemistry performed on formalin fixed paraffin FPPE.