Recently, the Tea Research Institute of the Chinese Academy of Agricultural Sciences and the Shanghai Institute of Plant Physiology and Ecology of the Chinese Academy of Sciences have conducted research on the genome assembly and gene editing of the chromosome of the major pest of tea trees-Grey tea looper An important breakthrough was made. The related research results were published online in the well-known journal Molecular Ecology Resources under the title of”Chromosome-level genome reference and genome editing of the tea geometrid” (“Chromosome-level genome reference and genome editing of the tea geometrid”). The results of this study provide key genomic information for the genomics of the gray tea geometries and the ecological adaptability of the population and the host.
Grey tea looper is the most widely distributed and most harmful leaf-eating pest in tea gardens in my country. The study used the third-generation nanopore sequencing technology combined with the second-generation transcriptome data to obtain a nearly complete genome of 787.4 Mb, 31 chromosomes and 18,746 coding genes of the gray tea looper. The study detailed annotated gene families related to chemoreception and detoxification, compared with other lepidopteran homologous genes, and found that the gray tea looper P450 multifunctional oxidase CYP6 family has specific gene expansion, and its taste receptor genes also have a large number of genes The expansion of the family indicates that the gray tea looper has the potential to be polyphagous.
The research team also used CRISPR/Cas9 gene editing technology to successfully knock out the somite development regulatory gene Abd-A, and obtain a somite variant mutant for subsequent development of gray tea The research on the gene function of the looper provides the basis.
This research was funded by the Frontier Science Key Research Project of the Chinese Academy of Sciences and the Outstanding Youth Science Fund of the National Natural Science Foundation of China. Dr. Pan Yunjie and Dr. Fang Gangqi from Shanghai Institute of Plant Physiology and Ecology and Dr. Wang Zhibo from the Tea Research Institute of Chinese Academy of Agricultural Sciences are the co-first authors of the paper. Researcher Zhan Shuai from Shanghai Institute of Plant Physiology and Ecology and Researcher Xiao Qiang from our institute are the co-corresponding authors.
link to the original text:https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13385
Figure 1. The life history and ecological photos of the gray tea ruler (a) the life history of the gray tea ruler (b) the gray tea Looper larvae (c) Gray tea looper adult (d) Gray tea looper pest in the field
Figure 2. Schematic diagram of the horizontal genome assembly of the gray tea looper chromosome (a) The gray tea looper genome on 31 chromosomes Distribution characteristics (b) Comparison of chromosome level genomics between gray tea looper and silkworm
Figure 3. Somatomorph mutants obtained after knocking out the Abd-A gene of the gray tea looper< /div>