The number of experiments carried out on monkeys by Cambridge scientists has increased.
Cambridge University has published details on the number of animal experiments from 2013-16.
Most controversially, monkeys - marmoset and macacques - are being used in animal testing in the city, according to data compiled by the Home Office.
The experiments have previously been met with protest by animal rights campaigners in the city.
In 2013 a total of 36 monkeys were tested, 42 the following year, 59 in 2015 and 52 last year.
Mice made up the majority of animals tested at 154,000 in 2013 down to 133,000 last year.
Zebra fish were used 17,431 times last year up from 9,907 in 2013. Xenopus, also known as aquatic frogs, were subjected to testing 619 times last year down from 1,001 in 2013.
Sheep were used 148 times in 2016 and 155 times in 2013. Rats were experimented on 3,523 times last year down from 4,328 in 2013. Guinea pigs were used 56 times last year up from 31 in 2016.
No chickens were experimented on last year compared to more than 800 were used in the previous three years.
The scientists are using animals to find treatments for illnesses ranging from Parkinson’s disease to Multiple Sclerosis, grow ‘mini-livers’ and carry out stem cell research.
A Cambridge University statement said: “Research using animals is essential for understanding the biology that underpins health and disease in both humans and other animals.
“Without such research, we would have few of the modern medicines, antibiotics, vaccines and surgical techniques that we take for granted in both human and veterinary medicine.
“Animals are used to investigate fundamental biology, to model disease and to test potential new treatments before they are tested in humans. Animal research is only undertaken where there is no alternative.
“The majority of the animals we use are mice and zebrafish – they make up 97 per cent of all procedures at Cambridge.
“Where these species are not suitable, we use a small number of other animals, such as xenopus frogs, rats and sheep, as well as non-human primates, namely marmosets and macaques.
“Some of the work carried out is fundamental research, aimed at understanding how humans and animals develop and how our immune systems and brains work, for example.
“This knowledge is essential for underpinning our understanding of health and disease for both medical and veterinary purposes.
"Other work is aimed at tackling specific diseases, for example in helping us understand how Parkinson’s disease affects the brain and motor system and how it might be tackled, or in developing new treatments for autoimmune diseases such as type 1 diabetes and multiple sclerosis.”
The statement added: “Procedures that are regulated include modifying the genes of a protected animal if this has the potential to cause the animal pain, suffering, distress or lasting harm; for example, breeding mice with harmful genetic defects is a regulated procedure if the intention is to keep the animals produced beyond two-thirds of the way through their gestation period.
“This helps explain why the numbers of mice and zebrafish are so high: each offspring born is counted as a procedure.
“Whilst every attempt is made to minimise the number of procedures undertaken in research, there has been an overall increase over the last decade due to the use of genetically-modified (GM) mice. If these breeding figures were to be excluded, the total number of procedures carried out year upon year would decrease slightly.
“We believe that good science and good animal welfare go hand in hand. The UK has the most rigorous animal welfare regulations in the world, and we consider adherence to these regulations as a minimum and will continue to aim for the highest possible standards of animal care.
“We strongly agree with, and rigidly follow, the guiding principles emphasised by the Home Office on the need to refine protocols, keep the numbers of animals used to a minimum and replace the use of animals with other methods where possible.
“To this end, we encourage all staff involved in animal research and husbandry to continuously develop and improve on existing welfare standards, offering incentives to those that contribute, and rewarding those that are recognised by the laboratory animal welfare organisations.
“We are committed to refining, reducing and replacing the use of animals in research - known as the 3Rs. Animals are only used where no alternatives are viable. Cambridge scientists are also leading research looking at finding viable alternatives.
“For example, in 2014, Dr Meritxell Huch from the Gurdon Institute won the UK’s international prize for the scientific and technological advance with the most potential to achieve the 3Rs for work to grow 'mini-livers' from adult mouse stem cells.”