Now to begin, pour 50 mL of the sodium phosphate buffer solution with a pH of 6.84 into the 150 mL beaker. From here on out, the sodium phosphate buffer solution will be referred to as simply the buffer solution. Next, locate the indicator called bromothymol blue (0.04%) and add 20 drops to your 150 mL beaker. The solution should then appear green. Next, obtain a 5 mL serological pipet and thoroughly rinse it with the buffer solution, then discard the buffer solution into the 250 mL beaker.
Then 5 and 10 grams of salt were weighed using the weighing cup and the scale. The weighing cups of salt were poured into beakers B and C. B receiving 5 grams and C receiving 10 grams. As the hot plate was warming up the salt was stirred in using the stirrer. When the hot plate was ready to go we placed beaker C (with 10 grams of salt) onto the hot plate. Once it started boiling the temperature was taken and it was 102 degrees Celsius.
Obtain beaker filled with 250 mL of HCl and 5 pennies. 2. Get a pipette and remove 10 mL of that solution (HCl + Zn) and place it in a 100 mL beaker. 3. Add color indicator 4.
» 2 Bunsen burners »2 tripods » 2 wire gauzes » 2 × 150 mL beakers » 2 × 250 mL beakers for 70°C and 80°C water baths » 5 Styrofoam cups for unheated water baths » 8 thermometers 0–100°C » 16 test tubes in a rack » 10 mL measuring cylinder » junket tablet » 50 mL milk » Crushed ice » dropper » plastic spoon » marking pen » Distilled water Note: Thermostatically controlled water baths may be used if available. Method 1 Prepare half-filled water baths by mixing different amounts of tap water, boiling water and ice in the styrofoam beakers to give one of each temperature: 10°C, 20°C, 30°C, 40°C and 50°C. Maintain the 70°C and 80°C temperatures by placing water in the beakers on the Bunsen burners and adjusting with cold water as necessary. Alternatively, use thermostatically controlled water baths for the 30°C, 40°C, 50°C, 60°C, 70°C and 80°C experiments. Place thermometers in each water bath to record the actual temperature in each bath.
Paper chromatography can be used in separating amino acids and anions, RNA fingerprinting, and testing histamines and antibiotics. (Infromation received from sonic.org Purpose The purpose of this experiment was to separate the dyes that these markers are composed of and show how chromatography works. Materials * Four different markers (including one black permanent marker) * Rubbing alcohol or isopropyl alcohol * Coffee filters (2) * Tall glasses or plastic cups (2) * Pencil * Ruler * Tape * Table salt * Water * Measuring cups/spoons * Clean pitcher or 2-liter bottle Procedure 1. Your first task is to cut the coffee filter into a rectangle measuring three cm by nine cm. You will need 2 for this lab.
Twenty drops of bromothymol blue was also added to the 150mL beaker. The pH was then obtained using the Vernier pH probe and it read 6.68. The 5mL pipet was then used to transfer 5mL of the green solution to the three 50 mL beakers. A 100mL beaker was obtained and filled with 1.0 M HCl solution and the pipet was used to acquire 1mL of the 1.0 M HCl solution. The 1mL of HCl was then transferred to one of the 50mL beakers turning the color of the solution to yellow.
The samples don’t have to have the same mass as long as it’s between 0.3 and 0.4g. Add about 20mL of water and 3 drops of phenolphthalein indicator to each sample and allow the solid to dissolve. Prepare a 50mL buret for use by washing it, rinsing it with tap water, and rinsing it twice with distilled water. Finally rinse it twice with 5mL portions of your sodium hydroxide solution. Mount the buret on the ringstand and fill it above the zero mark with the prepared sodium hydroxide solution.
Materials and Methods To begin, .4001 grams of Na2HPO4 and .4081 grams of NaH2PO4 solid was added together into a clean, dry 150 mL glass beaker. Then, approximately 50 mL of distilled water was added to the mixture, and the phosphate solids were stirred until fully dissolved. The last ingredient added to the beaker was exactly 20 drops of the liquid .04% bromothymol blue solution. Using the Vernier pH probe, the initial pH of this soluton was found to be 7.10. After obtaining three clean, dry 50mL glass beakers (Labeled one of each “yellow,” “blue,” and “green.”) approximately 5.00 mL of the solution from the 150mL beaker was added to all three with a volumetric pipet.
HYPOTHESIS: I hypothesize that the more concentrated sugar solutions will cause a greater amount of osmosis to make each solution isotonic. MATERIALS AND METHODS: Materials: Scale or balance, 24” dialysis tubing, 4 transfer pipets, sugar, scissors, rubber bands, 4 coffee sups of same size, 250ml graduated cylinder, ruler, small sauce and & 3 clean containers (600mls or larger), Methods: 1. Four 6-inch pieces of dialysis tubing were cut and soaked in a coffee cup filled with tap water for 2 hours prior to your start time. While waiting, I prepared the three sugar solutions. A) Added 5 grams of sugar to the 250ml graduated cylinder and then added water up to the 250ml mark.