There are often friends who are very curious about the principle of luminoluminescence. Desheng also talked about it before. If you are interested, you can learn about it on the Desheng news page. I will not elaborate here. After understanding the principle of luminol's chemiluminescence, will you be curious about its luminescence detection method? What are the three major luminescence detection methods of luminol?
The three major luminescence detection methods of luminol are adding catalyst to accelerate luminescence, adding inhibitor for indirect determination, and indirect determination by coupling.
1. Add a catalyst to speed up the luminescence method:
Adding a catalyst is a commonly used method at present. Under normal circumstances, the chemiluminescence reaction of hydrogen peroxide and luminol luminescence system is very slow, but after adding some catalysts, the reaction becomes very rapid.
Someone here will ask whether the existence of these catalysts will affect the entire system. De Sheng wants to say that the presence of a catalyst means that at the end of the reaction, the nature and quantity of the catalyst remain unchanged, that is, relative to the entire reaction, the catalyst does not participate. At the same time, these catalysts can be detected at a certain concentration, and the impact on the entire detection is almost negligible.
At present, the main catalysts of luminol include some metal complexes and transition metal ions. The metal complexes mainly include hemoglobin and peroxidase; the transition metal ions mainly include Fe3+, Fe2+, Mn2+, Cr2+, etc. The catalyst that we often use in chemiluminescence immunoassay is peroxide, especially horseradish peroxidase. Some compounds can be immunoreacted with peroxidase-labeled antibodies, and then chemiluminescence assays can be performed with luminol to determine these compounds or antibodies. Such as digoxin, hepatitis B surface antigen, etc. are all detected and analyzed by this method.
2. Indirect determination method by adding inhibitor:
In addition to the catalyst’s acceleration of detection, some inhibitors were also found in the research. These organic compounds can inhibit the chemiluminescence of luminol, such as reducing compounds containing phenolic hydroxyl groups, which can react with oxidants during the reaction and reduce the oxidants. The concentration of, the intensity of chemiluminescence is reduced, so as to indirectly measure this kind of organic substances.
3. Over-coupling indirect measurement method:
Finally, the method Desheng will introduce is to perform indirect measurement through coupling reactions. Coupling reactions here refer to combining one reaction that can produce or consume chemiluminescent reactants with another chemiluminescent reaction, so that certain substances can be realized. Indirect chemiluminescence determination. For example, some substrates produce hydrogen peroxide under the action of certain enzymes and then produce chemiluminescence with luminol. By measuring chemiluminescence, the purity of the measured substrate can be indirectly known.
Desheng is a high-tech enterprise focusing on the R&D and production of chemiluminescence, blood collection tube additives, biological buffers and color substrates. It has formed independent intellectual property rights and professional production research and development capabilities in blood collection tube additives. Provide products and raw material solutions for more than 100 domestic and foreign manufacturers. Chemiluminescence products include luminol and 6 types of acridinium esters.
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