GIS data used in creating this site’s maps are available in several formats.
For the global terrestrial maps, an ArcGIS Map Package can be downloaded here and for USA maps here. Map Packages require ArcGIS version 10.0 with service pack 1 or higher.
Zip files with GeoTIFFs/shapefiles for the global terrestrial maps can be downloaded here and for the USA maps here.
For the Brazil maps, GIS data are available on Dryad at https://dx.doi.org/10.5061/dryad.6rv61
For marine biodiversity, GIS data are available on Dryad at https://dx.doi.org/10.5061/dryad.3mn1t
A description of the methods used to prepare the biodiversity maps can be found here.
As a complement to the biodiversity data, the Natural Earth site is a good source for GIS layers on political and biophysical features.