iCLIP-seq (individual-nucleotide resolution UV crosslinking and immunoprecipitation) is a method used to identify protein-RNA interactions. This method uses ultraviolet light to covalently bind proteins and RNA molecules, with less background than RNA immunoprecipitation. As with all other methods of CLIP, iCLIP to connect the purification of proteins-RNA compounds is very strict to use immune precipitation, SDS-page, and then transferred to nitrocellulose membrane, finally remove radioactive marker protein-RNA complexes, RNA is released by using protease treatment in RNA crosslinking sites leave one or two amino acids.
Fig 1 Overview of the iCLIP experiment.
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 Busch Anke, Brüggemann Mirko, Ebersberger Stefanie, et al. iCLIP data analysis: A complete pipeline from sequencing reads to RBP binding sites [J]. Methods, 2020, 178:49-62.
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